Doctor's Data Inc

Hormones; saliva

Saliva testing is an easy and noninvasive way of assessing your patient's need for hormone balancing, and is proving to be the most reliable medium for measuring hormone levels. Unlike serum or urine testing, salivary hormone testing measures bioavailable hormones, the fraction that is actually delivered to receptors in the body. Clinically, it is far more relevant to test these bioavailable hormones, which provide an accurate reflection of the body’s active hormone levels. Learn more »


Turnaround Time

3 to 5 business days

Salivary Hormone Profiles

Click on any test name to learn more.

Comprehensive Plus Profile

Comprehensive Plus Profile

The Comprehensive Plus Profile expands on the Comprehensive Profile and includes estrone (E1), estriol (E3), and the Estrogen Quotient (EQ). Henry Lemon MD developed the Estrogen Quotient, a simple ratio of the cancer protective E3 relative to the proliferative estrogens E1 and E2, to assess breast cancer risk. A lower number (<1.0) indicates increased risk, and a higher number (>1.0) signifies lower risk. Dr. Lemon stated that for maximum protection, an optimal EQ is >1.5. This information may be extrapolated to provide information on other estrogen driven disorders. Because the research on the EQ focused on women, no reference range has been established for males, however some health care providers appreciate the information the additional estrogens provide in relation to prostate health.

This test is useful for:

  • Research indicates this profile could be considered for those with increased risk of developing breast cancer, or history of breast cancer or other hormonally sensitive cancers.
  • Personal or family history of autoimmune disease
  • PCOS

E1, E2, E3, PG, T, D, CX4

Women's Health & Breast Profile

Women's Health & Breast Profile

A comprehensive preventative breast health program extends beyond imaging and lifestyle optimization, and includes accurate assessment and comprehensive treatment of demonstrated hormone imbalances. The Women’s Health and Breast Profile is an accurate, non-invasive tool to advance risk prevention and support you in providing exceptional care to your patients.

The Women’s Health and Breast Profile includes two risk assessment ratios, the Estrogen Quotient and the Pg/E2 ratio. Dr. Henry Lemon developed the Estrogen Quotient (EQ) which is a simple ratio of the cancer protective E3 to the proliferative estrogens E1 and E2. According to Dr. Lemon, the EQ provides an assessment of breast cancer risk, with a lower number (<1.0) indicating increased risk, and a higher number (>1.0) signifying a lower risk. Dr. Lemon’s work stated that for maximum protection, an optimal EQ is >1.5. The Pg/E2 ratio, developed by Dr. John Lee, illuminates the relationship between estradiol, which is a proliferative hormone, and progesterone, which balances estradiol and slows cellular proliferation. The information gained from this profile will help to further the conversation with your patients about targeted treatment options such as BHRT, nutraceutical and herbal supplementation, as well as potential lifestyle modifications.

This test is useful for:

  • Research indicates this profile could be considered for those with increased risk of developing breast cancer or history of breast cancer or other hormonally sensitive cancers
E1, E2, E3, PG, T, D, CX4

Comprehensive Hormone Profile

Comprehensive Hormone Profile

The Comprehensive Hormone Profile is the best option for baseline testing of male or female hormonal status. This non-invasive test requires only 2cc per vial of saliva in 4 separate collections. Sex hormones are tested, as well as 4 cortisol levels to evaluate the diurnal cortisol pattern. This test is a consideration in men and women concerned with changing hormone levels as a result of age, cycling women experiencing PMS, Peri- and post-menopausal women concerned with their estradiol and progesterone levels for replacement considerations, and anyone with symptoms involving fatigue, insomnia, stress, immunity problems, blood sugar problems, and obesity.

This test is useful for:

Women Experiencing:

  • Hot flashes
  • Night sweats
  • Breast tenderness
  • Irritability
  • Forgetfulness
  • Irregular menstrual cycles
  • Vaginal dryness
  • Urinary incontinence
  • Uterine fibroids
  • Increased facial / body hair
  • Acne

Men Experiencing:

  • Decreased libido
  • Erectile dysfunction
  • Loss of stamina
  • Decrease in mental sharpness
  • Reduced muscle size
  • Increased moodiness
  • Metabolic syndrome
  • Prostate enlargement or cancer
  • Hot flashes
  • Irritability

Men or Women experiencing:

  • Weight gain
  • High blood sugar
  • Elevated lipids (cholesterol and/or triglycerides)
  • Insomnia
  • Fatigue
  • Fibromyalgia
  • Decreased stamina
  • Anxiety/Depression
  • Chronic disease
E2, Pg, T, D, Cx4

Short Comprehensive Profile

Short Comprehensive Profile

This assessment is useful in males and females whose primary symptoms are related to sex hormone imbalances (elevated or depressed E2, Pg, or T). The abbreviated adrenal profile that includes DHEA and the AM30 and PM cortisol levels provides a brief assessment of the level of HPA axis dysfunction. This profile is often used for re-evaluation 2-3 months after hormone replacement has been initiated to monitor therapeutic values.

This test is useful for:

Women Experiencing:

  • Hot flashes
  • Night sweats
  • Breast tenderness
  • Irritability
  • Forgetfulness
  • Irregular menstrual cycles
  • Vaginal dryness
  • Urinary incontinence
  • Uterine fibroids
  • Increased facial / body hair
  • Acne

Men Experiencing:

  • Decreased libido
  • Erectile dysfunction
  • Loss of stamina
  • Decrease in mental sharpness
  • Reduced muscle size
  • Increased moodiness
  • Metabolic syndrome
  • Prostate enlargement or cancer
  • Hot flashes
  • Irritability

Men or Women experiencing:

  • Weight gain
  • Insomnia
  • Fatigue
  • Anxiety/Depression
E2, Pg, T, D, Cx2

Basic Hormone Profile

Basic Hormone Profile

The Basic Hormone Profile provides a basic evaluation of the sex hormones and a brief glimpse at HPA axis function with the AM30 cortisol level. This profile is often used for re-evaluation 2-3 months after hormone replacement has been initiated to monitor therapeutic values.

This test is useful for:

Women Experiencing:

  • Hot flashes
  • Night sweats
  • Breast tenderness
  • Irritability
  • Forgetfulness
  • Irregular menstrual cycles
  • Vaginal dryness
  • Urinary incontinence
  • Uterine fibroids
  • Increased facial / body hair
  • Acne

Men Experiencing:

  • Decreased libido
  • Erectile dysfunction
  • Loss of stamina
  • Decrease in mental sharpness
  • Reduced muscle size
  • Increased moodiness
  • Metabolic syndrome
  • Prostate enlargement or cancer
  • Hot flashes
  • Irritability
E2, Pg, T, D, C

Comprehensive Adrenal Function Profile

Comprehensive Adrenal Function Profile

This salivary hormone profile provides a comprehensive view of the stress response and HPA axis/adrenal function and includes 4 cortisol levels collected over the course of the day, as well as a secretory IgA level.

This test is useful for:

  • Feeling stressed
  • Fatigue
  • Insomnia
  • Nervousness/Irritability
  • Salt/Sugar cravings
  • Dizzy spells
  • Headaches
  • Decreased stamina
  • Burn out
  • Chronic disease
  • Anxiety/depression
D, Cx4, sIgA

Adrenal Function Profile

Adrenal Function Profile

This profile provides a comprehensive view of HPA axis/adrenal function and includes 4 cortisol levels collected over the course of the day, plus DHEA.

This test is useful for:

  • Feeling stressed
  • Fatigue
  • Insomnia
  • Nervousness/Irritability
  • Salt/Sugar cravings
  • Dizzy spells
  • Headaches
  • Decreased stamina
  • Burn out
  • Chronic disease
  • Anxiety/depression
D, Cx4

Diurnal Cortisol Profile

Diurnal Cortisol Profile

This profile provides a comprehensive view of HPA axis/adrenal function and includes 4 cortisol levels collected over the course of the day. Samples are collected 30 minutes after waking, before lunch, before dinner, and before bed.

This test is useful for:

  • Feeling stressed
  • Fatigue
  • Insomnia
  • Nervousness/Irritability
  • Salt/Sugar cravings
  • Dizzy spells
  • Headaches
  • Decreased stamina
  • Burn out
  • Chronic disease
  • Anxiety/depression
Cx4

Cortisol Awakening Response (CAR)

Cortisol Awakening Response (CAR)

The cortisol awakening response is the natural rise in cortisol that is seen 30 to 40 minutes after awakening followed by a noticeable drop by 60 minutes. CAR can be utilized as a biomarker for assessment of the HPA axis function in routine clinical practice. CAR is influenced by overall HPA reactivity, as well as a person’s anticipation of stress.

The CAR involves a measure of the percent rise in cortisol from awakening to 30 minutes post-awakening and the expected decline in cortisol seen at 60 minutes. The Diurnal Cortisol Profile involves 4 cortisol samples taken at 30 minutes post-awakening, noon, dinner time, and bedtime. While the CAR can provide the best information on HPA axis reactivity and function, the Diurnal Cortisol Profile can identify dysregulation in the natural cortisol circadian rhythm.

This test is useful for:

  • Feeling stressed
  • Fatigue
  • Insomnia
  • Nervousness/Irritability
  • Salt/Sugar cravings
  • Dizzy spells
  • Headaches
  • Decreased stamina
  • Burn out
  • Chronic disease
  • Anxiety/depression
Cx3

Melatonin Profile

Melatonin Profile

The Melatonin Profile provides a snapshot of the sleep/wake cycle during a one day period. This profile (also can be added on to any saliva profile) is a consideration for those experiencing sleep disturbances.

This test is useful for:

  • Difficulty falling asleep
  • Difficulty staying asleep
  • Fatigue
Mx3

Hormones: Estrone (E1), Estradiol (E2), Estriol (E3), Progesterone (Pg), Testosterone (T), DHEA (D), Cortisol (C), Melatonin (M), Secretory IgA (sIgA)


Analytes Tested

Click any analyte name for additional clinical information, including reference ranges, specimen collection, stability and rejection criteria.


Analyte CPT ABN Required
Estrone; saliva 82679 Yes
Estradiol; saliva 82670 Yes
Estriol; saliva 82677 Yes
Progesterone; saliva 84144 Yes
Testosterone; saliva 84402 Yes
DHEA; saliva 82626 Yes
Secretory IgA; saliva 82784 Yes
Cortisol; saliva 82530 Yes
Melatonin; saliva 83520 Yes

List price applies when filing with insurance or Medicare, or when billing a patient directly.

Prompt payment pricing applies when billing to a physician account or prepayment is received with the test.

Doctor's Data offers profiles containing multiple analytes. *Multiple analytes may be billed under a single CPT code. Many analytes can be ordered individually. Pricing may vary. Click on a specific analyte for more information or read our detailed billing and payment policies.

The CPT codes listed on our website are for informational purposes only. This information is our interpretation of CPT coding requirements and may not necessarily be correct. You are advised to consult the CPT Coding Manual published by the American Medical Association. Doctor's Data, Inc. takes no responsibility for billing errors due to your use of any CPT information from our website.

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This test is useful for:

  • Males and females concerned with age related hormone changes
  • Cycling women experiencing PMS symptoms
  • Peri- and post-menopausal women concerned with erratic cycles, hot flashes and night sweats, vaginal dryness, low libido, and bone loss
  • Males concerned with low libido, decreased erections, BPH, loss of muscle mass, burn out, and bone density
  • Those wishing to monitor hormone levels following replacement therapy (oral, sublingual, topical, injectable, pellet)
  • Anyone with symptoms involving mood concerns, fatigue, insomnia, stress, immunity problems, blood sugar problems, cognitive concerns, and an overweight body should consider testing for cortisol levels as well as "sex" hormones

Detailed Information

Hormones are powerful molecules essential for maintaining physical and mental health. Estrogen is commonly thought to be a female hormone and testosterone important for males, but males and females need both, plus many other hormones that need to be in balance for optimal health. An imbalance of any combination of hormones can throw physical and mental health out of balance, causing aggravating and even serious health problems.

One size does not fit all when it comes to hormones. For decades, Western medicine has prescribed hormone replacement therapy as if everyone needed the same thing, in same amount. Nothing could be further from the truth. Hormones are like fingerprints; in order to achieve optimal health, a patient’s individual imbalances must be identified. Salivary hormone testing can help identify these imbalances.

There are several ways to test for hormones (saliva, serum and urine), but saliva testing is considered to be the most accurate way to assess bioavailable hormone levels. Hormones are lipophilic as they have a cholesterol backbone. Because of this, they must be bound to carrier proteins when travelling through serum, which renders them inactive. Urine reflects hormone metabolites, and is the best medium for assessing the way the body is metabolizing and excreting hormones. Bioavailable hormones are not readily assessed in either medium, but hormones found in saliva are bioavailable. This free fraction is reflective of tissue levels of hormones, and is therefore the best method to assess hormone imbalances that are actively contributing to health and a patient’s symptom picture. In addition, if using a topical (transdermal) or sublingual hormone preparation for treatment, saliva testing is the most accurate tool to measure and monitor hormone status.

Why Use Saliva?

Saliva testing is proving to be the most reliable medium for measuring hormone levels. Hormone levels in saliva accurately represent the amount of hormone delivered to receptors in the body, unlike serum which represents hormone levels that may or may not be delivered to receptors of the body. Clinically, it is far more relevant to test the amount of hormones delivered to the tissue receptors, as this is a reflection of the active hormone levels of the body. The majority of hormones in the blood exist in one of two forms: free (5%) or protein bound (95%). While 95% of the hormones in the body are protein bound, it is only the 5% free hormones that are biologically active. Therefore, serum is a much less accurate measurement than that of saliva when assessing functional hormone levels.

Saliva measures the "unbound" biologically active or free hormone levels in the body:
When blood is filtered through the salivary glands, the bound hormone components are too large to pass through the cell membranes of the salivary glands. Only the unbound hormones pass through and into the saliva. What is measured in the saliva is considered the "free", or bioavailable hormone, that which will be delivered to the receptors in the tissues of the body.

Serum measures the "protein bound" biologically inactive hormone levels in the body:
In order for steroid hormones to be detected in serum, they must be bound to circulating proteins. In this bound state, they are unable to fit into receptors in the body, and therefore will not be delivered to tissues. They are considered inactive, or non-bioavailable.

Only saliva testing measures topically dosed hormones:
The discrepancy between free and protein bound hormones becomes especially important when monitoring topical/transdermal or sublingual hormone therapy. Studies show that this method of delivery results in increased tissue hormone levels (thus measurable in saliva), but no parallel increase in serum levels. Therefore, serum testing cannot reliably be used to monitor topical hormone therapy.

For a male or female hormone test, reach out to Doctor's Data.

Endocrinology

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