Doctor's Data Inc

Zonulin Family Protein; stool

Elevated fecal levels of a zonulin family protein (ZFP) have been associated with metabolic syndrome and obesity; conditions that have been linked to increased intestinal permeability ("leaky gut"). Elevated serum levels of the ZFP antigen have been correlated with results from the established lactulose mannitol test. Definitive research has yet to be published regarding a correlation between fecal ZFP levels and the results of the lactulose mannitol test.

Learn more »

Turnaround Time

4 to 6 days

Analytes Tested

Click any analyte name for additional clinical information, including reference ranges, specimen collection, stability and rejection criteria.


Analyte CPT ABN Required
Zonulin Family Protein; stool 83520 Yes

List price applies when filing with insurance or Medicare, or when billing a patient directly.

Prompt payment pricing applies when billing to a physician account or prepayment is received with the test.

Doctor's Data offers profiles containing multiple analytes. *Multiple analytes may be billed under a single CPT code. Many analytes can be ordered individually. Pricing may vary. Click on a specific analyte for more information or read our detailed billing and payment policies.

The CPT codes listed on our website are for informational purposes only. This information is our interpretation of CPT coding requirements and may not necessarily be correct. You are advised to consult the CPT Coding Manual published by the American Medical Association. Doctor's Data, Inc. takes no responsibility for billing errors due to your use of any CPT information from our website.

Sign in at the top of any page to view pricing and order tests. Or click here to create an account. You may also contact us for assistance placing an order.

Detailed Information

A yet to be conclusively identified zonulin family protein (ZFP), possibly properdin, modulates the reversible breakdown of tight junction protein complexes (TJP) between gastrointestinal epithelial cells. Loss of integrity of the epithelial barrier permits paracellular influx of highly antigenic and pro-inflammatory polypeptides and other macromolecules from the lumen of the intestine. High serum levels of ZFP (antigen) are highly correlated with abnormal results of the Lactulose Mannitol test; the long-accepted standard for intestinal permeability.  Elevated fecal levels of ZFP have been associated with metabolic syndrome, obesity, and apparently healthy cigarette smokers. Elevated serum levels of zonulin have been correlated with results from the established lactulose mannitol test, but to date no such correlation has been reported with fecal zonulin. Excessive intake of simple sugars, sodium, emulsifiers, microbial transglutaminase ("meat glue") and nano-particles may also be triggers for increased fecal ZFP and intestinal permeability. Clinically the key is to first eliminate exposure to the trigger(s) of excessive ZFP. Possible interventions to restore the gastrointestinal mucosal barrier include dietary changes, treatment of dysbiosis, digestive supports and anti-inflammatory supplements; specifically quercetin, vitamin C, curcumin, gamma-linoleic acid, omega-3 fatty acids (EPA, DHA), and aloe vera. other nutrients such as zonc, beta-carotene, pantothenic acid, and L-glutamine may provide some support for rejuvenation of the mucosal barrier. The use of some probiotics has been show to reduce serum and fecal zonulin levels, and unulin (about 10 grams per day) lowered serum zonulin after just five days in healthy young subjects. Consider a comprehensive Stool Analysis to further investigate porential causes of increased intestinal permeability.

Gastrointestinal Health

Find out more